Med. Weter. 68 (8), 483-487, 2012

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Gryzińska M., Krauze M., Strachecka A.
Ca(2+)-ATPase activity according to the sex and age of Polbar hens
The aim of this study was to analyse the physiological values of Ca+2-ATPase catalytic activity in the muscles of 8-, 12- and 18-week-old Polbar breed at chickens, allowing for their sex. The material for the analysis was provided in the form of actomyosin homogenate sampled from the greater pectoral muscles (Musculus pectoralis maior) of 20 Polbar hens and 20 Polbar cocks, divided into three groups of 8-, 12- and 18-week-old birds. In the studies which were conducted the dependence of the calcium ATPase activity level on the sex and age of the examined birds was determined. Among the three age groups the highest ATPase activity was observed in the birds in their 8th and 12th weeks of life. In particular: in the case of the cocks the highest level of the activity was determined for the 8th week, with the mean value of 0.37 U/mg and in the case of the hens for 12th week of age, the mean value amounting to 0.39 U/mg. The obtained results for the ATPase activity in a genetically consolidated hen breed can be used for the comparison of other poultry breeds. The preliminary studies to establish reference values helped broaden the diagnostic range. Ca(2+)-ATPase, Polbar breed, skeletal muscle
Key words: Ca(2+)-ATPase, Polbar breed, skeletal muscle