Medycyna Wet. 64 (4b), 571-574, 2008
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| Wasielak M., Kamińska K., Bogacki M. |
| Effect of estradiol, progesterone and interleukin-1β on prostaglandin E2 and F2 secretion by porcine corpora lutea in vitro during the periimplantation period |
| This study examines the influence of ovarian steroids and interleukin-1β on prostaglandin E2 (PGE2) and F2α (PGF2α) synthesis and secretion from corpora lutea (CL) slices obtained from ovaries of gilts on days 12-14 of pregnancy and days 12-14 of the estrous cycle. For this study the authors used a surgically-generated model in which one of the uterine horns was cut transversely and a in separated part of this horn embryos did not develop. Collected corpora lutea were cut into slices and treated for 6 h with estradiol (E2) (100 nM/ml), progesterone (P4) (50 nM/ml) and interleukin-1β (IL-1β) (2.5 ng/ml). Results indicated that P4 increased PGE2 synthesis in CL slices originating from the ovary ipsilateral to the uterine horn with the developing embryos. P4 did not influence PGF2α synthesis in cultured CL slices from the ovaries of pregnant gilts regardless of embryos present in the uterine horn. In contrast to the effect of P4, E2 increased PGF2α synthesis in cultured CL slices from the ovary ipsilateral to the uterine horn with the developing embryos. E2 had no effect on PGF2α content in CL slices from the contralateral ovary of pregnant gilts and CL slices from both ovaries of cyclic gilts but increased PGF2α release from CL slices from the ipsilateral ovary of pregnant gilts. IL-1β markedly increased PGE2 release from cultured CL slices from the ovary contralateral to the uterine horn with the developing embryos from pregnant gilts and cyclic gilts regardless of CL origin. |
| Keywords: prostaglandins, corpus luteum, early pregnancy |