Medycyna Wet. 67 (2), 125-128, 2011
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| Rola J., Socha W., Żmudziński J.F. |
| Determining the genotype of bovine herpesvirus 1 strains isolated from cattle in Poland |
| The aim of this study was to determine the subtype of bovine herpesvirus 1 (BHV1) strains currently circulating in the cattle population in Poland. Altogether 224 nasal swabs, 36 lung tissue samples and 14 samples of fluid after embryo washing in cows were tested by virus isolation test. Five field strains of BHV1 were isolated. These isolates as well as 4 archival and 2 reference strains of BHV1 were subjected to restriction enzyme analysis. Viral DNA was cleaved with Hind III, Hpa I and Pst I enzymes. After digestion with Hind III typical patterns for BHV1.1 subtype were observed in all field strains. Among archival strains 2 belonged to BHV1.1 and 2 others to B-HV1.2a subtype. These findings were confirmed by digestion with other enzymes. The presented study showed that strains of BHV1.1 subtype are dominant in cattle in Poland |
| Keywords: cattle, bovine herpesvirus type 1 (BHV1), restriction enzyme analysis |