Medycyna Wet. 66 (9), 614-617, 2010
| Krukowski H., Lisowski A., Szymankiewicz M. |
| Intensity of slime production by yeast strains isolated from bovine mastitis cases and their susceptibility to polyenes |
| The purpose of this study was to determine the intensity of slime production by yeast-like fungi isolated from cases of bovine mastitis. Yeast isolates were recovered from 105 quarters of 87 cows with clinical and subclinical mastitis from 30 dairy herds in Poland over the period of October 2004 February 2010. Slime production was detected using a modified Christensen macrotube method established for coagulase-negative staphylococci. The adherent slime layer was scored as negative (), weak positive (1+), moderate positive (2+) or strong positive (3+). Out of 105 strains of yeast causing udder inflammation in cows, 22 (about 21%) were able to produce slime. 10.47% of yeast-like fungi isolated from bovine mastitis cases were able to form slime at the intensity level of 1+, 9.52% at the intensity level of 2+, while one strain (0.95%) produced slime at the intensity level defined as strong positive (3+). It was demonstrated that 22.34% of Candida genus strains were slime positive, while 40% of Geotrichum genus strains showed this activity. Yeast-like fungi of the genus Trichosporon did not produce slime. In an in vitro amphotericin B susceptibility assay only 9.63% of the 83 non-slime-producing (NSP) and 4.54% of the 22 slime-producing (SP) isolates were susceptible, 34.93% NSP and 13.63% SP isolates were moderately susceptible, whereas 55.42% non-slime-producing and 81.81% slime-producing strains were resistant to amphotericin B (p £ 0.05). 20 (24.09%) non-slime-producing and 1 (4.54%) slime-producing strains of yeast were susceptible to nystatin (p £ 0.05), 49 NSP (59.03%) and 17 SP (77.27%) fungi were moderately (adequately) susceptible, 14 NSP (16.87%) and 4 SP (18.18%) isolates were resistant to nystatin. Our studies indicate that slime-producing yeast strains isolated from the bovine mammary gland are more resistant to amphotericin B and less susceptible to nystatin in comparison with non-slime-producing strains. |
| Keywords: yeast, slime production, mastitis, susceptibility, polyenes, cows |