Medycyna Wet. 62 (11), 1261-1264, 2006
| Salwa A., Kopczewski A., Wolańczyk-Rutkowiak K. |
| Serologic and molecular examination of dogs vaccinated against parvovirosis |
| The aim of the study was to conduct serological examinations for the presence of humoral antibodies against CPV-2, using an ELISA test. Moreover, amplification and restriction analysis (PCR-RFLP) of the fragment at 1278 bp (VP-2 gene) strains of CPV-2 biological materials of dogs with diarrhea were performed. The studies were carried out on 377 urban dogs aged from 3 months 17 years. All animals were vaccinated with commercially available live or inactivated vaccines against canine parvovirosis at 8, 12, 16 weeks of age. Most of the dogs were revaccinated yearly. Serological examinations determined that most of the dogs had antibodies against CPV-2 (98%) at 2-3-years-of-age. The least seropositive dogs were below 5 months (89%) and above 10 years (85%). The highest mean titer CPV-2 virus antibody were found between 0.5 1 year. 95% animals with diarrhea were positive for canine parvovirosis by use of PCR. Moreover, the RFLP analysis of the VP-2 gene sequence enabled the distinction of 3 restriction patterns of CPV-2 circulating in the dog population. The study indicates the vaccination of dogs provides effective protection against canine parvovirosis infection. The occasional occurrence of CPV-2 in puppies and young dogs can indicate the presence of virulent strains of CPV-2 in the dog population. |
| Keywords: dogs, CPV-2, serology, PCR-RFLP |